abstract：:Force spectroscopy has been used to measure the adhesion of Saos-2 cells to a glass surface at different phases of the cell cycle. The cells were synchronized in three phases of the cell cycle: G(1), S, and G(2)M. Cells in these phases were compared with unsynchronized and native mitotic cells. Individual cells were a...

journal_title：Biointerphases

authors： Weder G,Vörös J,Giazzon M,Matthey N,Heinzelmann H,Liley M

更新日期：2009-06-01 00:00:00

abstract：:The authors report on the characterization of the charge formation at supported bilayer lipid membranes (sBLMs) prepared from the zwitterionic lipid 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine on planar silicon dioxide substrates. The charging of the sBLMs was studied in KCl solutions of different ionic strengths be...

journal_title：Biointerphases

authors： Zimmermann R,Küttner D,Renner L,Kaufmann M,Zitzmann J,Müller M,Werner C

更新日期：2009-01-01 00:00:00

abstract：:Dodecin from Halobacterium salinarum is a dodecameric, hollow-spherical protein, which unspecifically adopts flavin molecules. Reduction of flavin dodecin holocomplexes induces dissociation into apododecin and free flavin. Unspecific binding and dissociation upon reduction were used as key properties to construct an e...

journal_title：Biointerphases

authors： Grininger M,Nöll G,Trawöger S,Sinner EK,Oesterhelt D

更新日期：2008-09-01 00:00:00

abstract：:Simulations of water at hydrophilic self-assembled monolayer (SAM) surfaces are especially relevant for biological interfaces. Well-defined, atomically smooth surfaces that can be continuously varied are possible with SAMs. These characteristics enable more accurate measurements than many other surfaces with the added...

journal_title：Biointerphases

authors： Stevens MJ,Grest GS

更新日期：2008-09-01 00:00:00

abstract：:Supported phospholipid bilayers are interesting model systems for biologists and present fascinating physical properties. The authors present an extensive experimental study of the dynamic properties of supported bilayers. The structure and the equilibrium properties of single and double supported bilayers were invest...

journal_title：Biointerphases

authors： Charitat T,Lecuyer S,Fragneto G

更新日期：2008-06-01 00:00:00

abstract：:Solid-supported bilayer lipid membranes (SBLMs) containing membrane protein have been generated through a simple lipid dilution technique. SBLM formation from mixtures of native Escherichia coli bacterial inner membrane (IM) vesicles diluted with egg phosphatidylcholine (egg PC) vesicles has been explored with dissipa...

journal_title：Biointerphases

authors： Dodd CE,Johnson BR,Jeuken LJ,Bugg TD,Bushby RJ,Evans SD

更新日期：2008-06-01 00:00:00

abstract：:The authors investigate membrane composition-mediated interactions between proteins adsorbed onto a two-component lipid bilayer close to critical demixing using coarse-grained molecular dynamics simulations and a phenomenological Ginzburg-Landau theory. The simulations consist of three-bead lipids and platelike protei...

journal_title：Biointerphases

authors： Reynwar BJ,Deserno M

更新日期：2008-06-01 00:00:00

abstract：:The authors report a facile method for the selective immobilization of biomolecules onto a gold surface that was preactivated by a polymeric adlayer. The polymeric adlayer was designed to perform triple functions: high resistance to nonspecific protein adsorption, efficient surface anchoring, and subsequent covalent a...

journal_title：Biointerphases

authors： Lee BS,Park S,Lee KB,Jon S,Choi IS

更新日期：2007-12-01 00:00:00

abstract：:Peptide nucleic acids (PNAs) are mimics of oligonucleotides containing a neutral peptidelike backbone and are able to bind complementary DNA targets with high affinity and selectivity. In order to investigate the effect of the ionic strength of the buffer solution, hybridization experiments with PNAs as (catcher) prob...

journal_title：Biointerphases

authors： Park H,Germini A,Sforza S,Corradini R,Marchelli R,Knoll W

更新日期：2007-06-01 00:00:00

abstract：:Living confluent fish fibroblast cells RTG-P1 from rainbow trout adherent on diamond were examined by attenuated total reflection (ATR) infrared (IR) spectroscopy. In particular, IR spectra were recorded dynamically during the adsorption of the cells onto the diamond and during their biochemically induced structural r...

journal_title：Biointerphases

authors： Schmidt M,Wolfram T,Rumpler M,Tripp CP,Grunze M

更新日期：2007-03-01 00:00:00

abstract：:Reliable, direct "sample-to-answer" capture of nucleic acid targets from complex media would greatly improve existing capabilities of DNA microarrays and biosensors. This goal has proven elusive for many current nucleic acid detection technologies attempting to produce assay results directly from complex real-world sa...

journal_title：Biointerphases

authors： Lee CY,Gamble LJ,Grainger DW,Castner DG

更新日期：2006-06-01 00:00:00

abstract：:A theoretical framework is developed to analyze molecular bond breaking in dynamic force spectroscopy using atomic force microscopy (AFM). An analytic expression of the observed bond breaking probability as a function of force is obtained in terms of the relevant physical parameters. Three different experimental reali...

journal_title：Biointerphases

authors： Hanke F,Kreuzer HJ

更新日期：2006-03-01 00:00:00